1.大规模ENU化学诱变和突变体创制（Large-scale ENU mutagenesis and mutant generation）

本研究通过化学诱变进行猪全基因组水平的随机突变和表型分析，建立了较大规模的突变体，筛选到了影响骨骼发育、肌肉发生、血细胞再生、行为、毛色等猪重要性状的功能基因和突变家系，促进了猪在生物医学中的应用。

This study established a relatively large-scale mutant population in pigs through genome-wide random mutagenesis using ENU and subsequent phenotypic screening. Functional genes and mutant lineages affecting important traits such as skeletal development, myogenesis, hematopoiesis, behavior, and coat color were identified, thereby promoting the application of pigs in biomedical research.

大规模ENU化学诱变和突变体创制

2.基因组编辑体系的建立与应用（Establishment and Application of Genome Editing in Pigs）

建立了猪基因组高效的基因编辑体系，实现了大片段定点插入、敲除、点突变以及多基因同时编辑。通过基因编辑方法进行猪的基因组遗传修饰，为培育猪新品种提供了育种材料和分子标记，为构建生物医学研究的大动物模型的应用奠定了基础。

  This study established an efficient genome editing platform in pigs, enabling precise site-specific large-fragment insertions, gene knockouts, point mutations, and multiplex gene editing. This platform provides valuable genetic resources and molecular markers for the development of improved pig breeds and disease models.

高效的猪基因组编辑体系的建立与应用

pUCP1-KI基因编辑猪的构建和米色脂肪形成和发育的机制解析